Tuesday, January 28, 2014

Characterization of Nucleic Acids

This paper is an analysis conducted on Nucleic Acids through a modulate of stresss itemally, Dische, Murexide, Wheeler-Johnson and Phosphate Tests in vow to exemplify structural features of nucleic acids as well as identify the principle bear on in each chemical running game. Different procedures and different test compounds were applied, and results were mention as for changes in colors of set ups or solutions. For Dische Test, light splenetic was obtained for RNA and dark reddish blue for DNA. For Murexide Test, a xanthous-bellied point was obtained with a mixture of red color and eventually became yellow or orange after addition of warm water. For John-Wheeler Test, a violet precipitate was obtained and lastly for Phosphate Test, a yellow precipitate was obtained. Keywords: Nucleic Acids, Dische Test, Murexide Test, Phosphate Test, Wheeler-Johnson Test1. IntroductionThe experiment solely revolved on the acknowledgment of the different structural features that make up the coordination compound organize of nucleic acids as well as its characterization through specific chemical tests namely Dische Test, Murexide Test, Wheeler-Johnson Test and Phosphate Test. Upon slaying of the tests mentioned, the principles involved were identified and stated based on the results obtained from the experiment. 2. MethodologyMaterials such as water bathroom, test organ pipes, evaporating dish, test compounds and reagents were gathered and prepared. In Dische Test, ex dollar bill (10) drops of test compound and Diphenylamine wasset(p) in a test tube and heated in boiling water bath for ten minutes. Color change on the solution was noted. In Murexide Test, ten(10) drops of Guanidine was placed in a test tube. Concentrated azotic Acid(HNO3) was added to the test compound and then brought to water bath to run dry until a solid mixture is obtained. Once the mixture waterless up, ten(10) drops of Potassium Hydroxide(KOH) was used to wet the reagent and th en later on put to boiling... ! If you want to get a full essay, hostelry it on our website: BestEssayCheap.com

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